Title of article :
LIPOPROTEIN LIPASE mRNA EXPRESSION IN DIFFERENT TISSUES OF FARM ANIMALS
Author/Authors :
Elkattawy, Azza M. Kafr EI-Sheikh University - Faculty of Vet Med - Department of Biochemistry, Egypt , El-Shazly, Samir A. Kafr EI-Sheikh University - Faculty of Vet Med - Department of Biochemistry, Egypt , KAhilo, Khalid A. Kafr EI-Sheikh University - Faculty of Vet Med - Departmentof Biochemistry, Egypt , Hassan, Ibrahim F. Kafr EI-Sheikh University - Faculty of Vet Med - Department of Biochemistry, Egypt , Abo Zeid, Tarek K. Kafr EI-Sheikh University - Faculty of Vet Med - Department of Biochemistry, Egypt
Abstract :
Lipoprotein lipase (LPL) controls triacylglycerol partitioning between adipose tissues and muscles, so it is important enzyme for fattening of animals .The present work was planned to clarify the use of polymerase chain reaction (PCR) for detection of LPL mRNA expression in different tissues representing internal organs of male Friesian cows. The obtained results revealed that, LPL mRNA was expressed in all tested tissues, and the highest expression was encountered in testis (1.03). The expression in heart, liver, kidney, adrenal gland, spleen were 0.92, 0.77, 0.76, 0.73, and 0.70 respectively .The lowest expression was found in adipose tissue (0.64) It could be concluded from the present study that, expression of LPL mRNA differs according to the type of tissue. The highest expression was found in testis which may confirm the role of LPL in the process of spermatogenesis.
Journal title :
The Egyptian Journal of Biochemistry and Molecular Biology
Journal title :
The Egyptian Journal of Biochemistry and Molecular Biology
