Synthesis, Characterization, and In Vitro ‎Drug Release and In Vitro Antibacterial ‎Activity of O/W Nanoemulsions Loaded with ‎Natural Eucalyptus Globulus Essential Oil‎

Due to diverse biological activities, eucalyptus oil from eucalyptus waste leaves has tremendous applications in food and pharmaceutical industries. Since it is highly unable, hydrophobic and volatile and so easily loses its anti-microbial activity. To protect this herbal product having bioactive properties, nanoemulsion technology is the best way to encapsulate it. The objective of this study was synthesis of O/W type nanoemulsions, its characterization and evaluation of biological activities. O/W type eucalyptus oil based nanoemulsions were prepared by using sodium dodecyl sulphate, tween 20. Other physiochemical parameters viz; conductivity, pH, and antibacterial activity were also studied. UV, fluorescent and FT-IR techniques were also used. Drug release and pharmokinetic studies were also conducted. Stability of nanoemulsions was studied for 50 days. As compared to tween 20, SDS based nanoemulsions were smaller is size. Nanoparticles were spherical in shape and maximum encapsulation efficiency of 58%. Nanoparticles were stable even after 50 days of storage. Both nanoemulsions exhibited antibacterial and anti-inflammatory activities. Pharmokinetic studies revealed that formulated nanoemulsions followed korsmeyer-Peppas model. All these observations indicated that nanoemulsions from eucalyptus oil have key potential to act as key therapeutic agents in pharmaceutical and food based industries.