Title of article :
Identification of bovine leukemia virus in raw milk samples in North-West of Iran
Author/Authors :
Barzeghar ، Hossein Department of Microbiology and Virology - School of Medicine - Zanjan University of Medical Sciences , Mirshahabi ، Hessam Department of Microbiology and Virology - School of Medicine - Zanjan University of Medical Sciences , Motamed ، Nima Department of Social Medicine - School of Medicine - Zanjan University of Medical Sciences , Yavarmanesh ، Massoud Department of Food science and Technology - Ferdowsi University of Mashhad , Mahdavi Poor ، Behroz Department of Laboratory Sciences - Faculty of Paramedicine - Tabriz University of Medical Sciences , Moaddab ، Reza Department of Laboratory Sciences - Faculty of Paramedicine - Tabriz University of Medical Sciences , Asgharzadeh ، Mohammad Hematology and Oncology Research Center, Faculty of Paramedicine - Tabriz University of Medical Sciences
Abstract :
Bovine leukemiavirus (BLV) is one of the most important carcinogenic viruses genetically related to the human T-cell lymphotropic viruses (HTLV-1 and HTLV-2). The virus infects type B lymphocytes and creates lymph glands tumors. Recently, the association between the presence of this virus and breast cancer has been addressed in humans. Here, we studied the prevalence of BLV in the samples of raw milk of native Iranian and Iranianforeign cows in traditional, semiindustrial and industrial dairy farms in rural and urban areas of Zanjan province. Raw milk samples of cows were collected manually in sterile tubes. The samples were tested by nestedPCR method. Forty samples (9.93%) out of 403 samples showed BLV contamination. In this study, nested-PCR was successfully applied to determine the level of contamination in raw milk samples from cows infected with BLV. Furthermore, a relatively high rate of BLV infection was found in dairy cows in Zanjan province, northwestern of Iran.
Keywords :
Bovine leukemia virus , Breast cancer , Milk , Nested , PCR
Journal title :
Veterinary Research Forum
Journal title :
Veterinary Research Forum
