NOSOCOMIAL ACINETOBACTER INFECTION IN ASSIUT UNIVERSITY HOSPITAL

Aim:This study aimed to isolation of Acinetobacter spp, detect resistance patterns of isolated strains, phenotypic and genotypic detection of Metallo-β-lactamase and typing of isolated strains from clinical and environmental respectively. Material and method: this study includes 440 different clinical specimens, 672 environmental samples, inoculated on different culture media, confirmation of isolates by API20NE, PCR to detect 16SrRNA-23SrRNA gene, determine susceptibility pattern of isolates to different antibiotics and phenotypic and Genotypic detection of bla –OXA 51-like gene. Result: 24 strains of Acinetobacter (5.45%) were isolated from 440 clinical samples, 27 strains of Acinetobacter were isolated from 672 environmental samples (4.017%). Tetracycline was the most active drug against multi-drug resistant A.baumannii. (48/51 or 94%) of Acinetobacter isolates showed increase in zone of inhibition around IPM/EDTA disc compared with IPM disc alone. (49/51 or 96%) of Acinetobacter isolates were detected by presence of 16srRNA - 23srRNA gene (universal gene present in all Acinetobacter species). (49/51 or 96%) of isolated Acinetobacter spp showed band with blaOXA-51-like” genes. (37/51 or 72.5%) Acinetobacter isolates showed positive bands for class I integrase gene (gene responsible for multi drug resistance and outbreaks in hospitals).