Molecular Fingerprinting of Jojoba [Simmondsia chinensis (Link) Schneider] Clones with Inter-Simple Sequence Repeat (ISSR) Markers

Abstract. Jojoba (Simmondsia chinensis [Link] Schneider) is a crop ofhigh economic interest that is grown for the exceptional quality of itsseed oil as a medicinal and industrial vegetable oil. We have takenadvantage of the large variation commonly shown by inter simplesequence repeat (ISSR) to assess the genetic diversity among jojobaclones. The variation of DNA was analyzed with the use of an ISSRprimers generated by PCR. The number of amplification bands perprimer varied between 0 and 11. Analysis of the six ISSR primersamong jojoba clones in this study generated 41 bands. Over 85.4% (35bands) were found to be polymorphic in jojoba clones using ISSR.The genetic similarity coefficients among all jojoba ranged from 0.41to 0.71. The dendogram constructed with UPGMA analysis revealedtwo main clusters