Title of article :
The Effect of Vitamin E on the Expression of Mitochondrial Apoptotic Pathway genes in the Cerebellar Tissue of the Parkinson's Rat Model
Author/Authors :
Jamali, Behdokht Department of Molecular Cell Biology and Genetics - Faculty of Biology - Islamic Azad University, Bushehr , Entezari, Maliheh Department of Molecular Cell Biology and Genetics - Faculty of Biology - Islamic Azad University, Bushehr , Babaei, Nahid Department of Molecular Cell Biology and Genetics - Faculty of Biology - Islamic Azad University, Bushehr , Hashemi, Mehrdad Department of Molecular Cell Biology and Genetics - Faculty of Biology - Islamic Azad University, Bushehr
Abstract :
Introduction: Parkinson’s disease is one of the debilitating diseases which is more common
amongst the elderly; oxidative stress has been found to be one of its major causes. In this study,
therefore, the effect of vitamin E on the expression of Bcl-2 family genes was studied.
Materials and Methods: A total of 24 adult rats were purchased from the Pasteur Institute of
Iran. The rats were later divided into 4 groups: control group, control group receiving VitE, PD
group, PD group receiving VitE. After RNA extraction, cDNA synthesis, and primer design
of Bax and Bcl-2 genes, their expressions were studied in different rat groups by real-time
polymerase chain reaction (RT-PCR) technique.
Results:The mitochondrial apoptotic gene expressions were affected by vitamin E administration.
Vitamin E decreased the expression of Bax gene and overexpressed Bcl-2 gene.
Conclusion: Vitamin E plays an important role in reducing the effects of oxidative stress and,
consequently, apoptosis in the cerebellar tissue cells of rats with Parkinson’s disease
Keywords :
Rat , Bax , Bcl-2 , Parkinson’s disease
Journal title :
Archives of Advances in Biosciences
