Title of article :
Genetic Diversity Pattern of Marine Bacterial Strains Using Random Amplified Polymorphic DNA (RAPD) Technique
Author/Authors :
sabir, meshaal j. king abdulaziz university - faculty of science - department of biological sciences, Jeddah, Saudi Arabia , hajrah, nahid h. king abdulaziz university - faculty of science - department of biological sciences, Jeddah, Saudi Arabia , abo-aba, salah e. m. national research centre - microbial genetics department/ genetic engineering and biotechnology division, Giza, Egypt , abo-aba, salah e. m. king abdulaziz university - faculty of science - department of biological sciences, Jeddah, Saudi Arabia
Abstract :
A random amplified polymorphic DNA (RAPD) fingerprinting method has been used to fingerprint isolated marine bacterial strains. Six RAPD primers were used to differentiate 10 selected bacterial strains. Amplifications performed with primers UBC73, UBC88, UBC92, UBC96, UBC55 and UBC67 generate the best RAPD profiles. Dendrogram assessed by RAPD-PCR using six used primers yielded good banding patterns and showed that there is genetic diversity among strains. The RAPD profiles revealed a high level of DNA sequence diversity. Hence, this study, demonstrated that the local marine bacterial strains in this studied area are populated by genetically polymorphic strains. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the isolated bacterial strains.
Keywords :
Marine bacteria , RAPD , PCR , Dendogram , DNA
Journal title :
Journal of King Abdulaziz University : Science
Journal title :
Journal of King Abdulaziz University : Science
