Author/Authors :
Xu, Jian Engineering Center of Genetic Breeding and Innovative Utilization of Small Fruits of Jilin Province - College of Horticulture - Jilin Agricultural University, Changchun, PR China , Huo, Yile Engineering Center of Genetic Breeding and Innovative Utilization of Small Fruits of Jilin Province - College of Horticulture - Jilin Agricultural University, Changchun, PR China , Dong, Kun Engineering Center of Genetic Breeding and Innovative Utilization of Small Fruits of Jilin Province - College of Horticulture - Jilin Agricultural University, Changchun, PR China , Geng, Jinman Engineering Center of Genetic Breeding and Innovative Utilization of Small Fruits of Jilin Province - College of Horticulture - Jilin Agricultural University, Changchun, PR China , Dong, Mei Engineering Center of Genetic Breeding and Innovative Utilization of Small Fruits of Jilin Province - College of Horticulture - Jilin Agricultural University, Changchun, PR China , Tian, Youwen Engineering Center of Genetic Breeding and Innovative Utilization of Small Fruits of Jilin Province - College of Horticulture - Jilin Agricultural University, Changchun, PR China , Li, Yadong Engineering Center of Genetic Breeding and Innovative Utilization of Small Fruits of Jilin Province - College of Horticulture - Jilin Agricultural University, Changchun, PR China
Abstract :
Background: Cranberry (Vaccinium macrocarpon Ait.) has high developmental prospects and great research value.
Cranberry has a narrow genetic base, however, its morphological characteristics are not easily distinguishable. Besides,
traditional breeding methods are limited, and breeding progress on cranberry cultivars has been slow.
Objective: The objective of this study was to assess polymorphic EST-SSR markers developed from a cranberry fruit
transcriptomic sequencing library to provide candidate EST-SSR sequences for future research on stress resistance breeding
of cranberry.
Materials and Methods: Thirteen cranberry accessions were used for EST-SSR analysis, and 16 accessions of
other Vaccinium species were used to test primer transferability. Genomic DNA was extracted from young leaves of 6-yearold
cranberry plants and subjected to PCR amplification. A binary matrix was established and analyzed in NTSYS-pc v.2.10e
for calculation of the genetic similarity of cranberry cultivars and construction of a cluster dendrogram.
Results: A total of 47 stress-resistance-related primer pairs were designed, of which 7 pairs showed polymorphism. The
average number of effective alleles was 1.844, and the average expected heterozygosity was 0.455. The average transfer rate
was 63.39%. Genetic similarity coefficients ranged from 0.28 to 1.00, with an average of 0.76. UPGMA clustering divided
the 13 cranberry accessions into four groups at a genetic similarity of 0.74.
Conclusions: The seven polymorphic EST-SSR markers were able to reveal genetic relationships among 13 cranberry
accessions and can be used for future research on stress resistance breeding of cranberry.
Keywords :
Cranberry , EST-SSR markers , Genetic diversity , Transferability
