The Association Between Interleukin-10 Gene Polymorphisms and Hepatitis B Virus: Evidence in Iran

Background and aim: hepatitis b virus (hbv) is a human carcinogenesis agent. interleukin 10 (il-10) is a key anti-inflammatory cytokine, and single nucleotide polymorphisms in il-10 gene promoter are correlated with infections caused by hbv. this research intended to assess the prevalence and genotype of hbv as well as the association between the polymorphisms of -819 and -1082 in the il-10 gene with hbv in individuals with hbv infection in qom province, iran. methods: in this cross-sectional research, 360 individuals with chronic hbv infection and control group were involved between july 2018 and march 2019. hbv diagnosis was evaluated using elisa and nested pcr assays. to determine polymorphisms in the il-10 gene promoter in hbv positive and control samples, an allele-specific polymerase chain reaction technique was employed. results: the constructed phylogenetic trees for the hbsag gene revealed that all sequences under study belong to genotype d and also, the majority of hbv samples presented similar sequences to the iranian samples. genotype frequencies of tt, tc and cc (polymorphism -819) were 82.2%, 11.6% and 6.1% for patients and 85%, 10.5% and 4.4% in control groups, respectively. also, frequency of genotypes of aa, ag and gg (polymorphism -1082) were 45%, 43.8% and 11.1% for patients and 42.2%, 46.1% and 11.6% in control groups, respectively. conclusion: here, we found no association among il-10 gene polymorphisms in control and hbv-infected groups. however, more studies about the frequency of chronic hbv infection are necessary to be conducted.