Author/Authors :
Asgharzadeh, Mohammad Biotechnology Research Center and Faculty of Paramedicine - Tabriz University of Medical Sciences - Tabriz, Iran , Sanajou, Davoud Department of Clinical Biochemistry - School of Medicine - Tabriz University of Medical Sciences - Tabriz, Iran , Samadi Kafil, Hossein Drug Applied Research Center - Tabriz University of Medical Sciences - Tabriz, Iran , Farhoudi, Mehdi Neurosciences Research Center - Tabriz University of Medical Sciences - Tabriz, Iran , Savadi Oskouei, Daryoush Neurosciences Research Center - Tabriz University of Medical Sciences - Tabriz, Iran , Khaki-Khatibi, Fatemeh Department of Clinical Biochemistry - School of Medicine - Tabriz University of Medical Sciences - Tabriz, Iran , Ahmadi, Fatemeh Department of Biotechnology - Faculty of Advanced Medical Sciences - Tabriz University of Medical Sciences - Tabriz, Iran , Fadaee, Manouchehr Department of Immunology - Shiraz University of Medical Sciences - Shiraz, Iran , Vegari, A Department of Medical Physics - Faculty of Medicine - Urmia University of Medical Sciences - Urmia, Iran , Asgharzadeh, Vahid Hematology and Oncology Research Center - Tabriz University of Medical Sciences - Tabriz, Iran , Rashedi, Jalil Department of Laboratory Sciences - Faculty of Paramedicine - Tabriz University of Medical Sciences , Mahdavi Poor, Behroz Department of Laboratory Sciences - School of Paramedicine - Tabriz University of Medical Sciences - Tabriz, Iran , Gholizadeh, Pourya Hematology and Oncology Research Center - Tabriz University of Medical Sciences - Tabriz, Iran
Abstract :
Changes in the expression of cytokines as the result
of the single nucleotide polymorphisms (SNPs), can affect the
incidence of multiple sclerosis (MS).
Objective: To investigate the relationship between the frequencies
of interleukin-10 (IL-10)-1082 A/G (rs1800896) and CCR5-delta32
genotypes and susceptibility to MS in the Iranian Azari population.
Methods: IL-10-1082 A/G SNP and the CCR5-delta32 were
genotyped in 152 patients suffering from MS and 242 healthy
non-relatives by allele specific-PCR and simple PCR methods,
respectively.
Results: The frequencies of AA (37.6%) and AG (55.9%) genotypes
of IL-10-1082 were significantly high in the control (P=0.021) and
MS patients (P=0.015), respectively, with no statistical difference
between these groups. There was no significant difference in the
CCR5 gene based on the possession of wild/wild and wild/del32
genotypes between MS patients and the control group. The del32/
del32 genotype was not seen in any of the investigated groups.
Tobacco (cigarettes and hookahs) consumption was higher among
the MS patients (P=0.004), and this has the potential to raise the risk
of MS in both the individuals and their family. However, it had no
significant relation with the frequency of different genotypes of the
IL-10-1082 and the CCR5.
Conclusion: Our finding conclude on possible role of AA genotype
of IL-10 -1082 as a protective factor in MS.
Keywords :
Polymorphism , Multiple Sclerosis , Interleukin-10 , Delta 32 , CCR5
