Author/Authors :
Firooziyan, Samira Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran , Osanloo,Mahmoud Department of Medical Nanotechnology - School of Advanced Technologies in Medicine - Fasa University of Medical Sciences, Fasa , Moosa-Kazemi, Hassan Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran , Basseri, Hamid Reza Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran , Mohammadzadeh Hajipirloo, Habib Medical Parasitology Department - School of Medicine - Urmia University of Medical Sciences, Urmia , Sadaghianifar, Ali Urmia Health Center - Disease Control Unit - Urmia University of Medical Sciences, Urmia , Amani, Amir Natural Products and Medicinal Plants Research Center - North Khorasan University of Medical Sciences, Bojnurd , Sedaghat, Mohammad Mehdi Department of Medical Entomology and Vector Control - School of Public Health - Tehran University of Medical Sciences, Tehran
Abstract :
Background: Extensive use of chemical larvicides to control larvae, has led to resistance in
vectors. More efforts have been conducted the use of natural products such as plant essential oils
and their new formulations against disease vectors. Nanoformulation techniques are expected to
reduce volatility and increase larvicidal efficacy of essential oils. In this study for the first time, a
larvicide nanoemulsion from the essential oil of Acroptilon repens was developed and evaluated
against Anopheles stephensi larvae under laboratory conditions.
Methods: Fresh samples of A. repens plant were collected from Urmia, West Azarbaijan Prov-
ince, Iran. A clevenger type apparatus was used for extracting oil. Components of A. repens
essential oil (AEO) were identified by gas chromatography–mass spectrometry (GC–MS). All
larvicidal bioassay tests were performed according to the method recommended by the World
Health Organization under laboratory condition. Particle size and the morphologies of all pre-
pared nanoformulations determined by DLS and TEM analysis.
Results: A total of 111 compounds were identified in plant. The LC50 and LC90 values of AEO
calculated as 7 ppm and 35 ppm respectively. AEO was able to kill 100% of the larvae in 4 days.
Conclusion: The nanoemulsion of AEO showed a weak effect on the larvar mortality. It may
therefore be suggested that this kind of nanoemulsion is not appropriate for the formulation as
a larvicide. It is important to screen native plant natural products, search for new materials
Keywords :
Acroptilon repens , Nanoemulsion , Larvicidal effect , Vector control , Anopheles stephensi
