Author/Authors :
Beigh, Akeel Faculty of Veterinary Sciences & Animal Husbandry - Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu & Kashmir, India , Darzi, Mohmommad Faculty of Veterinary Sciences & Animal Husbandry - Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu & Kashmir, India , Bashir, Samina Department of Biochemistry - Hamdard University, New Delhi, India , Dar, Parvaiz Faculty of Veterinary Sciences & Animal Husbandry - Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu & Kashmir, India , Ganai, Nazir Faculty of Veterinary Sciences & Animal Husbandry - Sher-e-Kashmir University of Agricultural Sciences and Technology, Jammu & Kashmir, India , Malik, Suhail Sher-i-Kashmir Institute of Medical Sciences Soura, Jammu & Kashmir, India , Bhat, Basharat Department of Bioinformatics - Hamdard University, New Delhi, India
Abstract :
Background: Cystic Echinococcosis (CE) is an emergent or re-emergent zoonosis and
remains a public health and economic problem all over the world.
Methods: The present study was carried on the prevalence and genotypes of Echinococcus
present in small ruminants in Kashmir valley. A total of 2100, sheep (2052) and goats (48),
slaughtered or spontaneously dead, from various areas of Kashmir valley were screened for
the presence of hydatidosis. In case of goat none of the cases were found positive for hydatidosis,
whereas, all the positive cases (85) were recorded in sheep only. The overall prevalence
of hydatidosis was 4.04%. The prevalence was higher in female sheep (5.46%) compared
to males (2.83%). Season-wise highest prevalence was in summer (4.55%), followed
by autumn (4.1%), spring (3.89%) and winter (2.5%).The liver was observed to be the most
frequently infected organ with relative prevalence of 61.17% followed by lungs
(38.82%).The rDNA-ITS1 fragment of positive samples was amplified with BD1 / 4S primers.
Results: The length of amplified fragment for all isolated samples was 1000bps. The products
obtained on PCR were digested with four restriction enzymes (Rsa 1, Alu 1, Msp 1 and
Taq1). Rsa 1, Alu 1, Msp 1 yielded identical fragments, 300 and 700 bp in sheep. TaqI restriction
enzyme had no effect on PCR product and after digestion; intact 1000bps fragment
was seen.
Conclusion: Phylogenetic analysis of ITS1 gene revealed that the common sheep strain
(G1) is the predominant genotype in sheep in Kashmir valley.
Keywords :
Cystic echinococcosis , Polymorphism , Restriction fragment length , Genotyping , Sheep
