Author/Authors :
Hedayatyanfarda, Keshvad Department of Physiology and Pharmacology - School of Medicine - Alborz University of Medical Sciences, Karaj, Iran , Bagheri Khoulenjanic, shadab Polymer and Color Engineering Department Amirkabir University of Technology (Tehran Polytechnic) Tehran, Iran , Abdollahifard, Mohammad Amin Department of Biology and Anatomical Sciences - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran , Amanie, Davar Department of Immunology - School of Medicine - Shahid BeheshtiUniversity of Medical Sciences, Tehran, Iran , Habibi, Behnam Department of Pharmacology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Zare, Fatemeh Department of Immunology - Faculty of Medicine - Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran , Asadirad, Ali Department of Pharmacology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Pouriran, Ramin Department of Pharmacology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran , Ziai, Ali Department of Pharmacology - School of Medicine - Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract :
In this study, we evaluated the effects of nanofiber and film polymers with doxycycline for
treating a wound in a diabetic rat model. 108 male rats were divided into six groups, the control
group, the diabetic control, and the groups were diabetic rats receiving different wound dressing. At
the 3rd, 7th, and 14th days, macroscopic/histologic imaging and tissue sampling were performed.
Tissues were analyzed for IL-1β, TNF-α, IL-10, TIMP-1, and MMP-2 by using ELISA. Dressings
of chitosan, polyvinyl alcohol, and doxycycline increased the rate of wound closure, the volume
of collagen, dermal, and epidermis. In addition, it increased the number of fibroblasts and basal
cell epidermis cells, vascular length, and decreased the number of neutrophil cells. Inflammatory
cytokines and MMP-2 were decreased, and anti-inflammatory IL-10 and TIMP-1 were increased.
It was ultimately attained that the combination of chitosan/ polyvinyl alcohol /doxycycline could
be a useful dressing for the healing of diabetic wounds
Keywords :
Chitosan , Doxycycline , Film , Nanofiber , Diabetic wound , Polyvinyl alcohol
