Author/Authors :
Coeto Neitzke-Abreu, Herintha Universidade Estadual de Maringá, Maringá, Brazil , Carlos Souza Caetano, Rhuan Universidade Federal da Grande Dourados, Dourados, Brazil , Rosi Reinhold- Castro, Kárin Universidade Estadual de Maringá, Maringá, Brazil , Sebastião da Costa Lima-Junior, Manoel Department of Immunology - Aggeu Magalhães Institute - Oswaldo Cruz Foundation (Fiocruz), Recife, Brazil , José Tenório dos Santos, Wagner Department of Immunology - Aggeu Magalhães Institute - Oswaldo Cruz Foundation (Fiocruz), Recife, Brazil , de Alcantara Nogueira de Melo, Gessilda Departament of Clinical Analysis and Biomedicine - Universidade Estadual de Maringá, Maringá, Brazil , Kenji Nakamura Cuman, Roberto Universidade Estadual de Maringá, Maringá, Brazil , Mara Alessi Aristides, Sandra Universidade Estadual de Maringá, Maringá, Brazil , Gomes Verzignassi Silveira, Thais Universidade Estadual de Maringá, Maringá, Brazil , Valdrinez Campana Lonardoni, Maria Universidade Estadual de Maringá, Maringá, Brazil
Abstract :
Background: We aimed to determine the cellular recruitment (leukocyte rolling
and adhesion) by which the Leishmania (Viannia) braziliensis, L. (Leishmania) amazonensis,
and L. (Leishmania) major species in the mesenteric microcirculation of
BALB/c mice.
Methods: Five experimental groups were considered: group 1 (L. braziliensis);
group 2 (L. amazonensis); group 3 (L. major); group 4 (control group with PBS);
group 5 (negative control group), analyzed 3, 6, 12, and 24 h after parasite inoculation.
Results: Infections by the different Leishmania species caused an increase in the
number of rolling leukocytes: L. braziliensis a peak at 6 h; L. amazonensis and L. major
a peak at 3 h. The Leishmania infections induced leukocyte adhesion: L. major
and L. amazonensis showed an increase after 3 and 6 h, respectively.
Conclusion: The kinetics of cellular recruitment in Leishmania infections, leading
to infection susceptibility or resistance, indicates that distinct mechanisms regulate
the initial response to Leishmania infection and determine its course.
Keywords :
Leishmania , Rolling , Cellular adhesion , Leukocyte
