Author/Authors :
kahroba, houman tabriz university of medical sciences - faculty of advanced medical sciences, molecular medicine research center, biomedicine institute - department of molecular medicine, tabriz, iran , samadi, nasser tabriz university of medical sciences - faculty of advanced medical sciences, molecular medicine research center, biomedicine institute, faculty of medicine - department of molecular medicine, department of biochemistry, tabriz, iran , mostafazadeh, mostafa tabriz university of medical sciences - faculty of medicine - department of biochemistry, tabriz, iran , hejazi, mohamad saied tabriz university of medical sciences - faculty of advanced medical sciences, molecular medicine research center, biomedicine institute, faculty of pharmacy - department of molecular medicine, department of pharmaceutical biotechnology, tabriz, iran , sadeghi, mohammad reza tabriz university of medical sciences - faculty of advanced medical sciences, research center for pharmaceutical nanotechnology, biomedicine institute - department of molecular medicine, tabriz, iran , hashemzadeh, shahryar tabriz university of medical sciences - liver and gastrointestinal diseases research center, tabriz, iran , eftekhar sadat, amir taher tabriz university of medical sciences - imam reza educational hospital, school of medicine - department of general and vascular surgery, tabriz, iran , karimi, abbas tabriz university of medical sciences - molecular medicine research center, biomedicine institute, tabriz, iran
Abstract :
introduction: exosomal micrornas (mirnas) are emerging diagnostic biomarkers for different types of cancers. we aim to detect gastric cancer (gc)-specific mirnas in serum exosomes with diagnostic potential. methods: a pair of 43 tumor and tumoradjacent tissue biopsies obtained from gc patients, also 5 ml peripheral blood (following 12h fasting) were collected from the same patients and healthy controls (hcs). qiagen mircury lna mirna focus pcr panel applied to screen differentially expressed onco-mirnas. the candidate mirnas with the highest fold changes proceeded for validation by qrt-pcr in individuals. results: we identified that exosomal mir-10a-5p, mir-19b-3p, mir-215-5p, and mir-18a-5p were significantly upregulated in gc patient’s exosomes in contrast to hcs exosomes, roc curve analysis indicated area under the roc curve (auc) of 0.801, 0.721, 0.780 and 0.736 respectively. the roc curve analysis for the combined signature of four exosomal mirnas indicated auc of 0.813. also, spearman s correlation coefficients indicated that the mirna expression is highly correlated between tumor and exosome. conclusion: herein, we specifically identified four mirnas in serum exosomes of gc patients for a diagnostic purpose which are directly associated with tumoral mirna expression profile
Keywords :
liquid biopsy , exomir , oncomir , stem , loop rt , pcr , stomach
