Differential Pulse Voltammetric Method for Determination of Acemetacin in Pharmaceutical Formulation using Glassy Carbon Electrode

A new differential pulse voltammetric method was developed for determination of the non-steroidal anti-inflammatory drug; acemetacin. Various experimental parameters were studied, namely; electrode type, pH of the used buffer and scan rate on the reduction and oxidation peaks of acemetacin. The drug responded only to glassy carbon electrode among the studied working electrodes with higher peak current and sensitivity in the favour of the reduction side. The results also revealed that acemetacin best assayed through measuring its reduction peak current when prepared in Britton Robinson buffer solution at pH 8 when scanned at rate of 16 mV/s. The proposed method presented a bimodal calibration curve where each segment showed strong linearity. The linearity ranges were 1-100 μM for the lower segment and 0.1-3 mM for the higher one with detection limit of 0.1 μM. The proposed method was successfully applied for determination of acemetacin in its pharmaceutical dosage form.