Title of article :
Concanavalin-A Shows Synergistic Cytotoxicity with Tamoxifen via Inducing Apoptosis in Estrogen Receptor-Positive Breast Cancer: In Vitro and Molecular Docking Studies
Author/Authors :
Elshal ، Mohamed F. Department of Molecular Biology - Genetic Engineering and Biotechnology Research Institute - University of Sadat City , Eid ، Norhan m. Department of Molecular Biology - Genetic Engineering and Biotechnology Research Institute - University of Sadat City , El-Sayed ، Ibrahim Chemistry Department - Faculty of Science - Kafrelsheikh University , El-Sayed ، Wael Genetics Department - Faculty of Agriculture - Beni-Suef University , Al-Karmalawy ، Ahmed A. Department of Pharmaceutical Medicinal Chemistry - Faculty of Pharmacy - Horus University-Egypt
From page :
76
To page :
85
Abstract :
Background: Tamoxifen (TAM) is the main treatment of estrogen receptor (ER)-positive breast cancer, however; its adverse effects and development of resistance hinder its use. Concanavalin A (Con A) is a mannose/glucose-binding lectin that has been reported to induce apoptosis in a variety of cell lines. Methods: The effects of Con A on TAM-induced cell death in ERα positive cell line (MCF-7) were elucidated to identify the potential underlying molecular mechanisms using in silico (molecular docking) and in vitro (cytotoxicity assay, cell cycle analysis, annexin V-FITC apoptosis assay, and reverse transcription and quantitative real time-PCR) techniques as well. Results: The results demonstrated that combined treatment with Con A and TAM reduced the expression of ERα, which showed clear synergistic effects on inhibiting the cell viability of MCF- 7 cells. Interestingly, the combined treatment induces G1 phase arrest and reduces cyclin D1 activity while increasing apoptosis and autophagy as indicated by decreasing the expression level of anti-apoptosis gene BCl-2 and increased apoptosis/autophagic gene BNIP3. Molecular docking was conducted to evaluate the binding affinity of Con A towards ERα, and it revealed its potential activity as an ERα antagonist. Our data further indicated that Con A administration increased the drug reduction index of TAM. Conclusion: Overall, our findings suggested that Con A could be used as an adjuvant agent with TAM to improve its effectiveness as an anticancer agent.
Keywords :
Lectins , Tamoxifen , Chemoresistance , MCF , 7 , Apoptosis , Combination therapy
Journal title :
Pharmaceutical Sciences
Journal title :
Pharmaceutical Sciences
Record number :
2707771
Link To Document :
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