Title of article :
Enzymatic Effectiveness of Alcoholic and Aqueous Extract of Salvia Officinalis in Mice Poisoned with Tetrachloride
Author/Authors :
Kadhim, R Department of Chemical - College of Science - Tikrit University - Tikrit, Iraq , Ali, N. H Department of Chemical - College of Science - Tikrit University - Tikrit, Iraq , Aziz Ibrahim, D Department of Chemical - College of Science - Tikrit University - Tikrit, Iraq
Abstract :
Regarding the antioxidant, anti-inflammatory, and antibacterial effects of Salvia officnalis (S. officinalis)
extracts and the use of medicinal herbs as an alternative to chemical drugs, this study aimed to evaluate the
enzymatic changes and reduction of hepatocyte damage in mice poisoned with carbon tetrachloride (CCl4) after
treatment with aqueous and alcoholic extract of Salvia officnalis. A total of 40 adult male mice were divided
into eight groups including six experimental, one negative, and one positive control group, which were exposed
to CCl4 at the concentration of 2.3 mg/kg. The active compounds in the alcoholic and aqueous extracts of S.
officinalis were obtained using high-performance liquid chromatography. Subsequently, S. officinalis extract in
100, 200, and 300 mg /kg doses were fed orally to mice for six days. The enzymes (GST, ALP, ALT, AST, and
MDA) were determined in mice serum. The study results showed that enzyme activity was significantly
decreased in the group treated with S. officinalis extract, and the concentration of 300mg/kg proved to be most
effective. In addition, it was indicated that the alcoholic extract had a higher effect than the aqueous extract,
which might be due to the greater amount of active compounds in the alcoholic extract. The improving effects
of S. officinalis can be attributed to the bioactive components with antioxidant properties that inhibit the
damaging effects of free radicals, chemical drugs, and tissue damage.
Keywords :
Salvia officnalis , Enzymatic effective , Alcoholic extract , Aqueous extract , CCl4
Journal title :
Archives of Razi Institute