Some aspects of immune response to lipopolysaccharides of Providencia rettgeri

LPS of P.rettgeri was extracted and purified in our previous study, the present study found that the injection of 150μg/ Kg of LPS (intramuscularly and subcutaneously) was able to stimulate humoral immune response at both systemic and mucosal levels which was detected by passive haemmaglutination test and the mean values of systemic antibody titer (6826.6) was higher than the mucosal (at appendix) antibody titer (512). Some cytokines concentrations such as IL-2 ,IL-4,IL-6 and IL-1ß were detected using ELISA kits. The study was observed that, the concentrations of IL-2 (3.21 pg/ml ) , IL-4(5.84 pg/ml ) and IL-6 (4831 pg/ml) were increased significantly in LPS immunized animals group compared with control group which were 2.05 , 2.34 and 3121.06 pg/ml for IL-2,IL-4 and IL-6 respectively, while the concentration of IL-1ß (115.7 pg/ml ) was decreased significantly in LPS immunized animal group than control group which was 177.24 pg/ml