Author/Authors :
Bayat ، Payam Department of Immunology and Allergy - Immunology Research Center, BuAli Research Institute, Faculty of Medicine - Mashhad University of Medical Sciences , Abnous ، Khalil Department of Medicinal Chemistry - School of Pharmacy - Mashhad University of Medical Sciences , Balarastaghi ، Soudabeh Department of Pharmacodynamics and Toxicology - School of Pharmacy - Mashhad University of Medical Sciences , Taghdisi ، Mohamaad Department of Pharmaceutical Biotechnology - School of Pharmacy - Mashhad University of Medical Sciences , Saeedi ، Majid Department of Pharmaceutics - Faculty of Pharmacy - Mazandaran University of Medical Sciences , Yazdian-Robati ، Reavan Molecular and Cell Biology Research Center, Faculty of Medicine - Mazandaran University of Medical Sciences , Mahmoudi ، Mahmoud Department of Immunology and Allergy - Immunology Research Center, BuAli Research Institute, Faculty of Medicine - Mashhad University of Medical Sciences
Abstract :
Objective(s): Several studies reported the apoptotic and lytic activity of melittin (Mel) in different tumor cells. In this study, a novel nano-complex was developed composed of AS1411aptamers, melittin and gold nanoparticle for the treatment of breast cancer cells. Materials and Methods: Gold nanoparticles (GNP) were synthesized using reduction of tetrachloroauric acid (HAuCl4). Melittin modified with cysteine and AS1411aptamer conjugated to the gold nanoparticle. Gel retardation assay was used to prove the formation of GNP-Mel-AS1411 complex. Physicochemical properties of complex were investigated by Dynamic Light Scattering (DLS). The cytotoxicity of Mel and GNP-Mel- AS1411 complex were evaluated in both MCF‐7 (target) and L929 (non-target) cells by the MTT assay. Results: The average size of GNP and GNP-Mel-AS1411 complex were 20 ± 2.5 and 270.5± 3.2 respectively. The results of MTT assay revealed that this nanocomplex was more cytotoxic in MCF‐7 (cell viability = 19% ± 2%) and less cytotoxic in L929 cells (cell viability = 73% ± 1.6%). Conclusion: The results of this study indicated that the gold nanoparticle-melittin-AS1411 complex had a potential value in cancer cell targeted delivery of melittin.
Keywords :
AS1411aptamer , Gold nanoparticle , Melittin , Nucleolin , Targeted therapy
