Author/Authors :
mahdizade, elahe university of tabriz - faculty of natural sciences - department of biology, tabriz, iran , mahdizade, elahe tehran university of medical sciences - faculty of pharmacy - department of toxicology and pharmacology, tehran, iran , baeeri, maryam tehran university of medical sciences - faculty of pharmacy, pharmaceutical sciences research center (psrc), institute of pharmaceutical sciences (tips) - department of toxicology and pharmacology, toxicology and diseases group, tehran, iran , hodjat, mahshid tehran university of medical sciences - dental research center, dentistry research institute, tehran, iran , rahimifard, mahban tehran university of medical sciences - pharmaceutical sciences research center (psrc), institute of pharmaceutical sciences (tips) - toxicology and diseases group, tehran, iran , navaei-nigjeh, mona tehran university of medical sciences - pharmaceutical sciences research center (psrc), institute of pharmaceutical sciences (tips) - toxicology and diseases group, tehran, iran , haghi-aminjan, hamed ardabil university of medical sciences - pharmaceutical sciences research center, ardabil, iran , moeini-nodeh, shermineh tehran university of medical sciences - pharmaceutical sciences research center (psrc), institute of pharmaceutical sciences (tips) - toxicology and diseases group, tehran, iran , hassani, shokoufeh tehran university of medical sciences - pharmaceutical sciences research center (psrc), institute of pharmaceutical sciences (tips), faculty of pharmacy - department of toxicology and pharmacology, toxicology and diseases group, tehran, iran , dehghan, gholamreza university of tabriz - faculty of natural sciences - department of biology, tabriz, iran , hosseinpour-feizi, mohammad ali university of tabriz - faculty of natural sciences - department of biology, tabriz, iran , abdollahi, mohammad tehran university of medical sciences - faculty of pharmacy, pharmaceutical sciences research center (psrc), institute of pharmaceutical sciences (tips) - department of toxicology and pharmacology, toxicology and diseases group, tehran, iran
Abstract :
exposure to certain environmental toxins has been shown to be associated with cellular senescence mainly through induction of oxidative stress and impact on cellular systems. acrylamide (acr) has raised worldwide concerns regarding the high risk of human dietary exposure to its hazardous effect. although there is ample evidence about the carcinogenicity of acr, limited studies have focused on its impact on cellular aging. the levels of β-galactosidase (sa-β-gal) activity, cell cycle distribution, and the expression of the senescence-associated gene and inflammatory markers were evaluated following exposure of embryonic fibroblast cells to acr. a significant elevation in sa-β-gal activity after exposure to different concentrations of acr was accompanied by a considerably increased level of reactive oxygen species and lipid peroxidation. acr-treated cells showed a noticeable decline in the total antioxidant capacity and thiol molecules. moreover, the expression of cellular senescence-related genes including p38, p53, and p21 significantly upregulated at the high concentration of 5 mm acr. acr also induced g0/g1 phase arrest in embryonic fibroblast cells. the current study results revealed that exposure to acr could enhance senescence response, contributing to increased oxidative stress and cellular damage.
