Author/Authors :
tabatabaei mirakabad, fatemeh sadat shahid beheshti university of medical sciences - cellular and molecular biology research center, school of advanced technologies in medicine - department of medical biotechnology, tehran, iran , hosseinzadeh, simzar shahid beheshti university of medical sciences - school of advanced technologies in medicine - department of tissue engineering and regenerative medicine, tehran, iran , abbaszadeh, hojjat allah shaid beheshti university of medical sciences - laser application in medical sciences research center, hearing disorders research center, loghman hakim hospital, faculty of medicine - department of biology and anatomical sciences, tehran, iran , zeighamian, vahideh tabriz university of medical sciences - faculty of advanced medical sciences - department of medical biotechnology, tabriz, iran , khoramgah, maryam sadat shahid beheshti university of medical sciences - cellular and molecular biology research center, tehran, iran , ghanbarian, hossein shahid beheshti university of medical sciences - cellular and molecular biology research center, school of advanced technologies in medicine - department of medical biotechnology, tehran, iran , ranjbari, javad shahid beheshti university of medical sciences - cellular and molecular biology research center, school of advanced technologies in medicine - department of medical biotechnology, tehran, iran , kazemi, bahram shahid beheshti university of medical sciences - cellular and molecular biology research center, school of advanced technologies in medicine - department of medical biotechnology, tehran, iran
Abstract :
nowadays, mesenchymal stem cells (mscs) are the most widely used cell sources for bone regenerative medicine. electrospun polyacrylonitrile (pan)-based scaffolds play an important role in bone tissue engineering due to their good mechanical properties, which could be enhanced by the presence of nanoparticles such as nanoclay. this study evaluated the in-vitro effect of different concentrations of nanoclay in surface characteristic properties of pan-based electrospun nanofiber scaffolds and the osteogenic differentiation ability of adipose-derived mesenchymal stem cells (ad-mscs). after electrospinning nanofibers, their structure were assessed through some characterization tests. then ad-mscs isolation and characterization were done, and the cell attachment and the biocompatibility were determined. finally, osteogenic differentiationrelated markers, genes, and proteins were studied. clay-pan25% electrospun nanofiber scaffold could support attachment, proliferation, and osteogenic differentiation of ad-mscs better than other groups. also, nanoclay could enhance the properties of pan-based scaffolds, such as fiber diameter, topography, surface charge, hydrophilicity, roughness, and degradation, as well as osteogenic differentiation of cells. as a result, clay-pan25% with the highest concentration of nanoclay was found as a promising biodegradable and cost-effective scaffold for osteogenic differentiation of ad-mscs.
Keywords :
nanoclay , polyacrylonitrile (pan) , topography , osteogenic differentiation , mesenchymal stem cell (mscs)
