Title of article
Measuring of free endotoxin in alum-precipitated vaccine of haemorrhagic septicaemia by limulus amebocyte lysate test
Author/Authors
Jabbari, AR Razi Vaccine and Serum Research Institute, Karadj - Department of Aerobic Veterinary Bacterial Vaccine, ايران , Moazeni Jula, GR Razi Vaccine and Serum Research Institute, Karadj - Department of Aerobic Veterinary Bacterial Vaccine, ايران
From page
83
To page
85
Abstract
Haemorrhagic septicaemia (HS) vaccine which is prepared in Razi Institute is used in endemic areas of Iran. Aluminum-hydroxide gel was used as adjuvant for preparing this vaccine. Post-vaccinal shock reactions were the main complaint after use of this vaccine. In a previous study, we could improve the vaccine by alum-precipitation Pasteurella multocida cells and removing the liquid phase. In this study, the amount of free endotoxin in aluminum-hydroxide and alum-HS vaccines was determined. It was found that endotoxin level was considerably decreased from 0.22 EU/ml to 0.03 EU/ml after alum-precipitation
Keywords
Haemorrhagic septicaemia vaccine , Endotoxin , Shock reaction , LAL test
Journal title
Iranian Journal of Veterinary Research (IJVR)
Journal title
Iranian Journal of Veterinary Research (IJVR)
Record number
2711037
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