Title of article :
Complete Nucleotide Sequences and Construction of Infectious Clones of Two Jordanian Isolates of Tomato Yellow Leaf Curl Virus
Author/Authors :
Al-Abdallat, Ayed M. University of Jordan - Dept of Horticulture and Crop Science, Jordan , Al-Debei, Hmoud University of Jordan - Dept of Horticulture and Crop Science, Jordan , Akash, Muhannad University of Jordan - Dept of Horticulture and Crop Science, Jordan , Misbeh, Samar University of Jordan - Dept of Horticulture and Crop Science, Jordan , Kvarnheden, Anders Uppsala Bio Center SLU - Dept of Plant Biology and Forest Genetics, Sweden
Abstract :
The complete nucleotide sequences of two Jordanian isolates of Tomato Yellow Leaf Curl Virus (TYLCV) were determined and compared with other reported TYLCV isolates. Sequence analysis revealed that one isolate belonged to the mild strain, TYLCV-Mld, while the other isolate belonged to the Israel strain, TYLCV-IL. Agrodrench inoculation of tomato (Solanum lycopersicum L.) plants with a 1.8-mer infectious clone of TYLCVIL[ JO:Ju:08] resulted in the development of severe symptoms, while tomato plants infected with a 1.8-mer infectious clone of the isolate TYLCV-Mld[JO:Ju:08] resulted in milder symptoms. The infectious clones can be an important tool for improved understanding of TYLCV-tomato interactions
Keywords :
Agrodrench , Begomovirus , Solanum lycopersicum L.
Journal title :
Jordan Journal of Agricultural Sciences
Journal title :
Jordan Journal of Agricultural Sciences
