Survey and Determination of Aflatoxin Levels in Stored Peanut in Sudan

Sixty samples of stored peanut kernels were collected from four different locations in Sudan namely Mayo city, Umbaddah city in Khartoum state, Al-Helalia city, and Al-Managel city in Al-Jazeera state. The kernels were examined for contamination with aflatoxin. All samples were subjected to microbiological analysis by culturing them on suitable growth medium Sabouraud s Dextrose Agar and chemical analysis by Thin-Layer Chromatography (TLC) technique. Thirty five samples (58.33%) gave positive readings with TLC technique, and in culture, Aspergillus flavus was isolated from twenty six samples (43.33%). The concentration of aflatoxin B1 in these samples were ranged from low to very high, in range of (17.57-404.00 µg/Kg kernel).