Author/Authors :
Monsef Esfahani, Hamidreza Department of Pharmacognosy - Faculty of Pharmacy - Tehran University of Medical Sciences, Tehran, Iran , Amirshahrokhi, Keyvan Department of Pharmacology and Toxicology - School of Pharmacy - Ardabil University of Medical Sciences, Ardabil, Iran , Mashmool, Atiyeh Department of Pharmacognosy - Faculty of Pharmacy - Tehran University of Medical Sciences, Tehran, Iran , Moridi Farimani, Mahdi Department of Phytochemistry - Medicinal Plants and Drugs Research Institute - Shahid Beheshti University, Tehran, Iran , Dehpour, Ahmadreza Department of Pharmacology - Faculty of Medicine - Tehran University of Medical Sciences, Tehran, Iran , Miran, Mansour Department of Pharmacognosy and Biotechnology - School of Pharmacy - Ardabil University of Medical Sciences, Ardabil, Iran
Abstract :
Salvia reuterana, commonly known as Maryam Goli Esfahani, is a member of the Labiateae family. In Iranian folk medicine, aerial parts of S. reuterana have been used as sedative and anxiety. Evaluation of various extracts of the plant for their analgesic activity revealed that treatment of mice with n-hexane extract (500 mg/kg, i.p.) significantly increased the latency time as compared to the control group. Fractionation of the hexane extract of S. reuterana led to the isolation of sclareol as the main compound (0.19% w/w). Column chromatography was used to isolation of compounds from S. reuterana and a spectroscopic method including NMR was used to identification of the isolated compound. Evaluation of the analgesic effect of sclareol using a hot plate, tail-flick, and formalin tests in mice confirmed the potent analgesic effect of sclareol as an effective compound of S. reuterana. These results showed that the n-hexane extract of aerial parts of S. reuterana and its main constituent sclareol showed significant analgesic activity in different rodent nociceptive behavioral tests.
Keywords :
Analgesic Activity , Formalin Test , Hot Plate Test , Salvia reuterana , Sclareol , Tail-Flick Test
