An Effective Method for DNA Extraction From Human Hair Samples

Introduction: In historical cases, mass disasters, missing person’s identification, and Archaeogenetic investigations, the bone, teeth, and hair samples are often the best and the only biological materials available for DNA typing. In the present study, an extremely effective protocol for DNA recovery of hair has been demonstrated. Materials and Methods: Obtaining genetic information from hair instance for DNA Typing includes the following steps: sterilization, DNA extraction, quality control, polymerase chain reaction (PCR) and profiling. After performing a new extracting ancient DNA (aDNA) from the hair, autosomal short tandem repeat (STR) analyses were repeated for each sample using the AmpFℓSTR® MiniFiler™ and Identifiler™ kits. Results: The extracted DNA was evaluated quantitatively and qualitatively. The qualitative analysis of the DNA which had been obtained by electrophoresis showed that the quality of the extracted samples was very appropriate and that electrophoresis bands were observed. Conclusions: The results of our experiment demonstrated that the Fast DNA Extraction of Hair (FDEH) method is both cheaper and faster than commercial kits. Moreover, this new method enhances DNA recovery from hair. Because of the simple protocol and high quality, this method can be utilized in medicine.