Production of L-Asparaginase from Aspergillus Niger using Agro Wastes By-Products in Submerged Fermentation Process

The project was carried out to obtain maximum yield of L-asparaginase from Aspergillus niger using by-products of agro wastes incorporated with organic salts in submerged fermentation process. The main objective of the project was to study the kinetic parameters of L-asparaginase productivity. After optimization maximum enzyme activity (2.83U/mL±0.065) was achieved using corn steep liquor as a substrate and with 4% inoculum, pH 6.5, 1% substrate concentration, 96 hrs fermentation time period and 1% glucose was used as additional supplement to the growth media to obtain better yield of L-asparaginase. This study showed that glucose concentration beyond 1% suppressed the enzymatic activity. From the results it can be concluded that L-Asparaginase production was optimized when cheap agro-waste products were used as a substrate at low concentrations and under acidic conditions. Its relative stability in acidic pH conditions make it ideal for applications in health care systems and pharmaceutical industry.