Author/Authors :
Nasir, Nor Atikah Husna Ahmad Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Department of Bioprocess Engineering, Malaysia , Asri, Nor Fadhilatul Shilla Mohd Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Department of Bioprocess Engineering, Malaysia , Zain, Nor Azimah Mohd Universiti Teknologi Malaysia - Faculty of Biosciences and Bioengineering - Department of Industrial Biotechnology, Malaysia , Suhaimi, Mohd Suardi Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Department of Bioprocess Engineering, Malaysia , Idris, Ani Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Department of Bioprocess Engineering, Malaysia
Abstract :
This paper presents preliminary research on immobilized Phanerochaete chrysosporium in PVA- alginate-sulfate beads to discolor textile effluents. It is an alternative technique from the current physico-chemicals. The main focus of this study was to determine the colour removal, Chemical oxygen deman (COD) removal and manganese peroxidase activity of the immobilized P.crysosporium. Immobilized P.crysosporium also confers advantages such as reusability and improved cell performance. Scanning electron microscope (SEM) was also performed to characterize the immobilization matrix. The immobilized results were compared with that of free cells. Immobilized cells were able to discolor 47.14% compared to free cells which recorded 10.78% colour removal. The COD removal of immobilized cell is more than 60% as compared to that of free cells, which could only reduced 30% of COD. Finally, the manganase peroxidase activities showed a slight difference between the immobilized and free cell at 0.15U/L and 0.13U/L respectively.
Keywords :
PVA , alginate , sulfate beads , Phanerochaete chrysosporium , COD , MnP , SEM
