Author/Authors :
Shams, Gholamhossein islamic azad university - Department of Chemical Engineering, ايران , Ismail, Hassan Fahmi Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Tissue Culture Engineering Laboratory, Department of Bioprocess Engineering, Malaysia , Chee, Teh Liam Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Tissue Culture Engineering Laboratory, Department of Bioprocess Engineering, Malaysia , Ali, M Abbas Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Department of Bioprocess Engineering, Malaysia , Abdul Majid, Fadzilah Adibah Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Tissue Culture Engineering Laboratory, Department of Bioprocess Engineering, Malaysia , Sarmidi, Mohamad Roji Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Institute of Bioproduct Development, Malaysia , Abdul Aziz, Ramlan Universiti Teknologi Malaysia - Faculty of Chemical Engineering - Institute of Bioproduct Development, Malaysia
Abstract :
Selective and efficient analytical methods are required not only for quality assurance but also for authentication of herbal formulations. In the present study, high-performance liquid chromatography (HPLC) detection was developed for chromatographic fingerprint analysis of the extract from grounded plant materials of Jamu Diabetes (JD), a well known herbal formulation in Malaysia. The bioactive markers rosmarinic acid, curcumin, cinnamaldehyde and andrographolide were identified from the methanol/water (80:20) extract of JD materials. The data presented in this study showed that the developed method is simple, sensitive and specific for simultaneous determination of the indicated marker compounds either qualitatively or quantitatively, and may be used as a fingerprint profile for the standardization of extractives or herbal medicines from herbal formulation JD materials.
Keywords :
Jamu diabetes , HPLC , fingerprint , bioactive markers
