Title of article :
Effect of Lutein-Rich Extract on Human Cancer Cells
Author/Authors :
Omar, Wesam Mostafa Biotechnology and Life Sciences Department - Faculty of Postgraduate Studies for Advanced Sciences - Beni-Suef University, Beni-Suef, Egypt , E. Ahmed, Amr Biotechnology and Life Sciences Department - Faculty of Postgraduate Studies for Advanced Sciences - Beni-Suef University, Beni-Suef, Egypt , Raslan, Mai Biotechnology and Life Sciences Department - Faculty of Postgraduate Studies for Advanced Sciences - Beni-Suef University, Beni-Suef, Egypt , El-Nesr, Khalid Pathology Department - Faculty of Veterinary Medicine - Beni-Suef University, Beni-Suef, Egypt , Moawad Ali, Mamdouh Biochemistry Department - National Research Centre, Cairo, Egypt , De Abdelmaksoud, Mohamed Biochemistry Department - National Research Centre, Cairo, Egypt , El Dahshan, Dina Clinical Pathology Department - Faculty of Medicine - Beni-Suef University, Beni-Suef, Egypt
Abstract :
Background: Lutein and its isomerzeaxanthin are safe natural compounds. They are
able to reduce the development of tumor and other chronic diseases. The objectives
of the current study was to examine the cytotoxicity of lutein isolated and purified
from alfalfa, safe and low-cost plant, on five different human cancer cell lines, namely
(MCF-7), (HepG2), (A549), (PC3), and (HCT116), as well as normal (HFB4) cells.
Method: We examined the cytotoxicity of lutein purified from Medicago sativa L
and evaluate its activity against human liver HepG2, breast MCF-7, lung A549,
prostate PC3, and colon HCT116 cancer cell lines using SRB assay in comparison
with doxorubicin as a reference drug.
Results: Results revealed that the tested extract could be a more promising anticancer
agent in the case of MCF-7 (IC50, 3.10±0.47 μg/ml) compared with standard drug
doxorubicin (IC50, 2.90±0.30 μg/ml). Moreover, the extract showed a moderate effect
on HepG2 (IC50, 6.11±0.84 μg/ml) versus doxorubicin (IC50, 2.90±0.30 μg/ml);
meanwhile, the extract showed no activity against A549, PC3, and HCT116 cells.
The results further revealed that the extract had no toxicity against the growth of
normal HFB4 cells versus doxorubicin.
Conclusion: Lutein-rich extract from alfalfa had a major antiproliferative role in
breast MCF-7 and liver HepG2 compared to doxorubicin.
Keywords :
Carotenoids , Medicago sativa L. , Alfalfa , Cytotoxicity , Anticancer
Journal title :
Middle East Journal of Cancer (MEJC)
