The effect of combined application of new dentin desensitizing agent and deproteinization on dentin permeability of different adhesive systems

Purpose: To evaluate the effect of dentin desensitization using casein phosphopeptide amorphous calcium phosphate paste; CPP-ACP, with and without deproteinization (10% sodium hypochlorite), on dentin permeability of two types of self-etch adhesives and a total-etch one. Materials and methods: 90 freshly extracted, sound human molars were sectioned into 1 mm thick dentin disks, and randomly divided into three equal groups (S1, S2, and S3); 30 specimens each, according to the dentin surface treatment, as follows: (S1) adhesive system {AS} only, (S2) CPP-ACP + AS, (S3) 10% NaOCl + CPP-ACP + AS. Each group was further divided into 3 divisions (D1, D2, and D3), 10 specimens each, according to the tested adhesive system (Solobond M, Clearfill SE and Futurabond DC) respectively. Dentin surfaces were treated Dentin permeability was measured at base line and after dentin treatment and storage under simulated pulpal pressure for 10 min. Results: CPP-ACP treatment significantly decreased the permeability of (S2D2) and (S2D3), but didn’t affect (S2D1). The combined NaOCl and CPP-ACP treatment decreased the permeability of the three tested adhesives significantly. Clinical significance: The combined application of 10% sodium hypochlorite and CPP-ACP paste may be recommended to improve the dentinal permeability before using dental adhesive systems.