Allelic prevalence of intron 3 insertion/deletion genetic polymorphism of DNA double-strand break repair gene XRCC4 in four healthy Iranian populations

Background and purpose: The X-ray cross-complementing group 4 (. XRCC4; OMIM: 194363),plays an important role in repair of DNA double-strand breaks via non-homologous end joining pathway. In order to find the allelic prevalence of an insertion/deletion polymorphism in intron 3 of XRCC4 (Ins/Del; rs28360071) among Iranian populations,the present study was carried out. Subjects and methods: The total study subjects consisted of 662,200,291,and 200 individuals from Shiraz (Fars province; belong to Persians),Abarku (Yazd province; belong to Persians),Tabriz (East Azerbaijan province; belong to Azaris),and Yasuj (Kohgiluyeh va Boyer-Ahmad province; belong to Lurs),respectively. Genotypic analysis of the Ins/Del XRCC4 polymorphism was detected by the PCR method. Results: The prevalence of the Del allele in Shiraz,Abarku,Tabriz,and Yasuj was estimated 44.4%,37.3%,53.6% and 45.0%,respectively. Tabriz (Azaris) showed the significant difference with other populations (χ2=20.06,df=1,P 0.001). Also there was a significant difference between Persians of Shiraz and Abarku (χ2=6.43,df=1,P=0.011). Conclusion: Although there is a significant heterogeneity between Iranian populations,the Del allele shows high prevalence among Iranian populations,which is much higher than the allelic prevalence among Asians. © 2015 The Authors. Production and hosting by Elsevier B.V.