Author/Authors :
Hasan Basri, I.K Department Center for Technology of Radiation Safety and Metrology Indonesia - National Nuclear Energy Agency - Jalan Lebak Bulus Raya No. 49. Jakarta, Indonesia , Barnard, S Public Health England - Centre for Radiation - Chemical and Environmental Hazards - Chilton - Didcot - Oxon OX11 0RQ, United Kingdom , Suvivan, V.A Department Center for Technology of Radiation Safety and Metrology Indonesia - National Nuclear Energy Agency - Jalan Lebak Bulus Raya No. 49. Jakarta, Indonesia , Rahardjo, T Department Center for Technology of Radiation Safety and Metrology Indonesia - National Nuclear Energy Agency - Jalan Lebak Bulus Raya No. 49. Jakarta, Indonesia , Nurhayati, S Department Center for Technology of Radiation Safety and Metrology Indonesia - National Nuclear Energy Agency - Jalan Lebak Bulus Raya No. 49. Jakarta, Indonesia , Rahajeng, N Department Center for Technology of Radiation Safety and Metrology Indonesia - National Nuclear Energy Agency - Jalan Lebak Bulus Raya No. 49. Jakarta, Indonesia , Lusiyanti, Y Department Center for Technology of Radiation Safety and Metrology Indonesia - National Nuclear Energy Agency - Jalan Lebak Bulus Raya No. 49. Jakarta, Indonesia , Tetriana, D Department Center for Technology of Radiation Safety and Metrology Indonesia - National Nuclear Energy Agency - Jalan Lebak Bulus Raya No. 49. Jakarta, Indonesia , Syukri, M M. Jamil Hospital - Jl. Perintis Kemerdekaan Padang, Indonesia , Purnami, S Department Center for Technology of Radiation Safety and Metrology Indonesia - National Nuclear Energy Agency - Jalan Lebak Bulus Raya No. 49. Jakarta, Indonesia , Hiswara, E Department Center for Technology of Radiation Safety and Metrology Indonesia - National Nuclear Energy Agency - Jalan Lebak Bulus Raya No. 49. Jakarta, Indonesia
Abstract :
Background: Medical workers are exposed to long-term low levels of ionizing radiation, which makes them vulnerable to DNA damage. There are potential occupational health hazards from radiation exposure in a large occupational segment of the population. Matherials and Methods: During this study, 69 blood samples were taken from 45 medical workers (including diagnostic
radiologists, radiographers, and cathlab nurses) and 24 nonmedical workers
as controls from three hospitals across Indonesia, and were analyzed for the
presence of DNA damage. Detection of γ-H2AX expression as a biomarker of
DNA DSB damage and the micronucleus assay were carried out by
immunofluorescence microscopy and Giemsa staining, respectively. Results:
The mean γ-H2AX foci index in workers was 0.02(0.00-0.24) and in control
was 0.02(0.00-0.12), micronucleus frequency of workers (5-30 per 1000 cells)
and control (12-29 per 1000 cells). The annual occupational dose of workers
was recorded as (0.01-1.12 mSv). There was no statistical difference in the
mean number of γ-H2AX foci and the micronuclei frequency between workers
and controls, but there was a small tendency toward correlation between
γ-H2AX foci and micronuclei frequency and the annual occupational dose of
workers. Conclusions: γ-H2AX foci have the potential to be used as a
biomarker to detect radiation-induced DNA DSB damage in workers caused by occupational dose exposure.
Keywords :
γ-H2AX , micronucleus , occupational dose exposure , medical radiation workers
