Study the Toll-like Receptor 2 and Dectin-1 in mice treated with beta‐glucans and infected with Salmonella Typhimurium

Objective: Toll-like receptors (TLRs) are essential components for the induction of innate immune responses in different tissues including the small intestine, spleen and mesenteric lymph nodes. We investigated the expression of TLR2 in these tissues in mice infected with Salmonella Typhimurium. In this study we have examined how dectin-1, a lectin family receptor for β-glucans, collaborates with TLR-2 in recognition of microbes. Methods :In this study two groups of mice were treated with the β- glucan (local and commercial extract) and infected with(1x10 6 CFU/ml) S. Typhimurium at different intervals group 1 at day zero and group 2 after seven days of the experiment .The immunohistochemistory assay has been performed to detect TLR-2 and dectine-1 in the tissues of small intestine, spleen and mesenteric lymph nodes of the mice; the principle of this assay was done according to the manufacturer instructions (U.S.biological) .