Antibacterial action of five type of honey sample against three common pathogens isolate from surgical wound

The aim of the present study was to assess the in vitro antibacterial activity of honey on certain potentially pathogenic bacterial isolates., antibacterial activities of five honey samples collected from abu- alyakdan apiarist were all tested against three clinical pathogens isolate from surgical wound such as Staphylococcus aureus and Klebsiella pneumoniae. These honey samples were compared with standard antibiotics like Ampicillin, Tetracycline, ciprofloxacin. The antibacterial activity was tested using Kirby-Bauer’s method for antibiotics and well diffusion method for honey samples. The honey samples were tested at concentrations of (25%, 50%, 75%) . The all sample of honey showed distinct bacterial inhibitory effect in vitro at honey concentrations of 50% and above on the various tested bacteria. No growth inhibition effect was observed at 25 % concentration of all honey type against Kl.pneumoniae except mountaineer and eucalyptus honey which showed more activity against the three tested isolat. The study shows that honey more active than antibiotics, has certain organisms sensitive to it, and provides alternative therapy against certain bacteria and is also shown to have antibacterial action against a broad spectrum of bacteria, both gram- positive and gram-negative bacteria.