Title of article :
Induction of Immunogenic Response in BALB/c Mice by Live and Killed Form of Recombinant Lactococcus lactis Displaying EG95 of Echinococcus granulosus
Author/Authors :
Ebrahimzadeh ، Fatemeh Department of Medical Biotechnology - Faculty of Medicine - Zanjan University of Medical Sciences , Shirdast ، Hoda Department of Medical Biotechnology - Faculty of Medicine - Zanjan University of Medical Sciences , Taromchi ، Amirhossein Department of Medical Biotechnology - Faculty of Medicine, Cancer Gene Therapy Research Center - Zanjan University of Medical Sciences , Talebkhan ، Yeganeh Biotechnology Research Center - Pasteur Institute of Iran , Haniloo ، Ali Department of Medical Parasitology and Mycology - Faculty of Medicine - Zanjan University of Medical Sciences , Esmaeilzadeh ، Abdolreza Department of Immunology - Faculty of Medicine, Cancer Gene Therapy Research Center - Zanjan University of Medical Sciences , Nedaei ، Keivan Department of Medical Biotechnology - Faculty of Medicine - Zanjan University of Medical Sciences , Mirabzadeh ، Esmat Department of Molecular Medicine - Biotechnology Research Center - Pasteur Institute of Iran
Abstract :
Background: CE is a zoonotic parasitic infection caused by Echinococcus granulosus worldwide and is associated with economic losses among livestock animals. EG95 is an immunogenic antigen from the E. granulosus. Lactococcus lactis has been prested as a safe vehicle for antigen delivery. The goal of this study was to design a novel L. lactis strain displaying EG95 as a vaccine delivery system. Methods: The eg95 encoding gene fragment fused to the M6 anchoring protein was cloned into the pNZ7021 vector, and L. lactis NZ9000 displaying recombinant EG95 was constructed. The expression of an approximately 32-kDa EG95 protein was confirmed by Western blotting and immunofluorescence analysis. The immune responses were evaluated in BALB/c mice immunized orally and subcutaneously with the live and killed recombinant L. lactis, respectively. Results: Total IgG level in mice immunized with heat-killed recombinant L. lactis (pNZ7021-eg95) significantly increased compared to the control group. sIgA was significantly higher in mice received live recombinant L. lactis (pNZ7021-eg95) compared to the control mice. Splenic lymphocytes from immunized mice represented the high levels of IFN-γ and the low-levels of IL-4 and IL-10. Conclusion: Our results indicate that immunization with EG95-expressing L. lactis can induce both specific humoral and cellular immune responses in mice. DOI: 10.52547/ibj.25.4.284
Keywords :
Echinococcus granulosus , Lactococcus lactis , Immunization , Vaccines
Journal title :
Iranian Biomedical Journal(IBJ)
Journal title :
Iranian Biomedical Journal(IBJ)
