Enhancing the Antibacterial and Anticancer Activity of Ciprofloxacin by Encapsulating into Shellac-Chitosan Nano Particles (CNPs)

This study demonstrates a preparation of a nanocarrier for drug delivery system which based on combining two natural materials shellac (SH) and chitosan (CH). The chitosan shellac NPs (SH-CH NPs) was loaded with ciprofloxacin drug to enhance its anticancer and antibacterial activity. The shellac – chitosan NPs (SH-CH NPs) size was measured using DLS and its size was58.8nm ± 3.4, while the morphology of the nano compound was identified using FESEM, TEM techniques. We diagnosed SH-CH NP synthesis by FTIR, HNMR and DSC techniques. The results of MTT test showed a high inhibition rate of 55.45% for A375 melanoma cells, and a low inhibition rate for normal cells (HFF) range (8.51%-32.93%)when incubated with Cipro-SH-CH NPs. Also, the results reinforced the possibility of using ciprofloxacin loaded on chitosan – shellac NPs as a promising nanocarrier with low cytotoxicity, and high activity as an antibacterial with a diameter of 40 mm with both type of positively and negatively charged bacteria.