Graphene Oxide/Chitosan Based Impedimetric Aptasensor Along with an Ester Linker for the Detection of Tetracycline

In the present study, an impedimetric aptasensor was designed based on a glassy carbon modified with nano composite of graphene oxide-chitosan along with 1-Pyrenebutyric acid–N-hydroxysuccinimide ester (Pry) linker to detect tetracycline antibiotic. After the modification of the electrode surface, the aptamer strands were immobilized on it, and it was then used for determination tetracycline concentration. Under optimum conditions, aptamer revealed a linear range of 1.0 × 10^-15 - 3.2 × 10^-7 with a detection limit of 3.2 × 10^-16 M for determination of tetracycline. Some advantages of this biosensor are being highly selective and sensitive for detection of tetracycline, not expensive and easy preparation. Also, this designed aptasensor was applied in the real samples of drug and serum solutions successfully. Moreover, it can be implied that this creates a basis for improvement in graphene oxide-based impedimetric biosensors.