Title of article :
Silencing of HOTAIR Induced Apoptosis in Human Colorectal Cancer Cells through Up-regulation of Bax and Down-regulation of Bcl2
Author/Authors :
Dashtbozorgi ، Reza Cellular and Molecular Research Center, Medical Basic Sciences Research Institute - Ahvaz Jundishapur University of Medical Sciences , Tahmasebi-Birgani ، Maryam Department of Medical Genetics - School of Medicine - Ahvaz Jundishapur University of Medical Sciences , Hajjari ، Mohammad-Reza Department of Genetics - Faculty of Sciences - Shahid Chmaran University of Ahvaz , Emami Razavi ، Amirnader Cancer Biology Research Center, Cancer Institute of Iran - Tehran University of Medical Sciences
Abstract :
HOX transcript antisense RNA (HOTAIR), as a long noncoding RNA (lncRNA) is a highly cited transcript modulating variety of signaling pathways such as cell growth and apoptosis. Altered expression of HOTAIR has been reported in human cancers, which contributes with cancer progression and metastasis. Increased expression level of HOTAIR has been observed in colorectal cancer (CRC). It seems that dysregulation of HOTAIR may inhibit the apoptosis. The present study was aimed to evaluate the effect of HOTAIR silencing on expression of apoptosis markers Bax and Bcl2 using real-time polymerase chain reaction (PCR). The data showed that HOTAIR and Bcl2 are highly expressed in CRC cells while the expression level of Bax is low. Following siRNA treatment, Blc2 was downregulated but Bcl2 was upregulated. These findings suggest that HOTAIR silencing can promote apoptosis, and thus it can be considered as a promising strategy to kill cancer cells.
Keywords :
Colorectal Cancer , Apoptosis , Long Noncoding RNA , HOTAIR , Bax , Bcl2
Journal title :
Jentashapir Journal of Cellular and Molecular Biology
Journal title :
Jentashapir Journal of Cellular and Molecular Biology
