Fourier Transform Infrared Spectroscopy as diagnostic tools for bladder cancer treatment: Doxorubicin and Cisplatin and Reduced Graphene Oxide as nanocarrier

Objective(s): The use of Fourier Transform Infrared Spectroscopy (FTIR) as diagnostic tools for bladder cancer associate to histopathology analysis. Methods: FTIR imagens of normal and bladder cancer tissues in rats o Brucker model V 70 spectrophotometer provided with the MCT detector (Mercury Cadmium Telluride), under attenuated total reflectance (ATR). Histopathology analysis of rat tissues and comparison of diagnostics in both methods. Results: All the spectroscopic parameters analyzed showing that the progress of cancer treatment were consistent with the histopathological analyses. The ratio of FTIR bands intensity of protein/lipid (I1633/I1743) was highly increased (ratio of 3.33) in the cancer tissues compared with normal one (ratio of 1.22). After treatment with DOX-CIS-rGO nanocarrier a value of protein/lipid ratio was 1.38. Conclusions: All data of the ATR-FTIR imagens will be important tools for the most complex and expensive histopathological analysis. The ATR-FTIR diagnosis will be an important data in the final decision related to progress in the chemotherapeutic treatment. Of course, the fast analysis by ATR-FTIR methodology compared to histopathology could be an important support for monitoring all these studies.