Preparation of Composites of Antibacterial ‎Materials Based on Bacterial Cellulose ‎and Silver Nanoparticles for Wound ‎Healing

This study examined the preparation of composites of Bacterial Cellulose (BC) / silver nanoparticles (Ag NPs) using a substrate for the fermentation of the bacteria Gluconacetobacter xylinus C3 and a reducing agent for the synthesis of silver nanoparticles in situ. The presence of silver in the BC/ Ag NPs composites was confirmed by various characterization tests. Thus, according to Scanning Electron Microscopy, the average size of silver was recorded at 700 nm. Xray phase analysis showed an increase in the size of cellulose crystallites up to 122 Å with a decrease in the amount of silver nanoparticles. According to UVvisible spectroscopy, an identification peak was found between 420580 nm. Measurements of the percentage of water absorption and the edge angles of wetting with water confirmed the structures of the resulting nanocomposites with a smaller pore diameter and a narrow pore distribution over the film size, depending on the silver content. Using the disk diffusion method, excellent antimicrobial activity was shown with a decrease in the number of bacteria in relation to the pathogenic microorganism Staphylococcus (S.) aureus, Pseudomonas (P.) aeruginosa, Escherichia (E.) coli.