Author/Authors :
Niknazar, Somayeh Hearing Disorders Research Center - Loghman Hakim Medical Center - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Abbaszadeh, Hojjat-Allah Hearing Disorders Research Center - Loghman Hakim Medical Center - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Forooghirad, Hosna Department of Biology - Payame Noor University (PNU) - Tehran, Iran , Shahbazi, Fatemeh Department of Biology - Payame Noor University (PNU) - Tehran, Iran , Peyvandi, Ali Asghar Hearing Disorders Research Center - Loghman Hakim Medical Center - Shahid Beheshti University of Medical Sciences - Tehran, Iran , Khoshsirat, Shahrokh Hearing Disorders Research Center - Loghman Hakim Medical Center - Shahid Beheshti University of Medical Sciences - Tehran, Iran
Abstract :
Background: Today, hearing disorders are one of the most common problems in an industrial society. High-intensity sounds can induce apoptosis and metabolic changes in the auditory neural pathway and auditory cortex.
Aim: In this study, we investigated the effect of chronic noise exposure on Bax, Bcl-2 and Caspase-3 genes expression in the auditory cortex of rats.
Methods: Chronic exposure to 110 dB white noise was applied in male rats for 6 hours for 5 consecutive days. Superoxide dismutase (SOD) activity was measured in the serum of rats prior to and following noise exposure. Bax, Bcl-2 and Caspase-3 genes expression in the auditory cortex was determined by Real time PCR assay.
Results: Our results showed that the serum SOD level was significantly decreased in rats exposed to noise. In addition, the gene expression of Caspase-3 and Bax/Bcl-2 ratio was markedly increased in the auditory cortex of rats were subjected to chronic noise exposure compared to control rats.
Conclusion: Chronic noise exposure can activate apoptosis signaling pathway in the auditory cortex of rat.
Keywords :
Apoptosis , Auditory Cortex , Bax , Bcl-2 , Caspase-3 , Chronic Noise Exposure
