Immune Responses in Patient Infected with Entamoeba histolytica and the Antigensity of Cyst in Basrah Province, Iraq

Aims Amoebiasis was a main parasitic infestation triggered via Entamoeba histolyticawith clinical signs assortments starting moderately to heavy. This study aimed to culture E. histolytica in vitro from the stool samples of humans, and the stage of the cyst was purified. Materials & Methods Stool plus blood samples were collected from patients attending Basrah Hospitals. The identification of E. histolytica was done by macroscopic and microscopical examination. The high efficiency was recorded in the culture medium for isolating E. histolytica from the stool samples of humans. The cysts were purified. Findings The purity by use of precoll gradient is higher than in the culture medium and the protein of E. histolytica cysts was prepared. The immunogenicity of E. histolytica cysts was considered by using SDS-PAGE and Western blot. Seven proteins had been valued in patient serum as more immunogenic, their molecular weight was (83, 69, 61, 34, 29.1, 18.1, and 11.2kDa). Also, the intensities of IL-4 and IFN-γ were assessed by using ELISA kits in the serum of amoebiasis patients. Conclusion This study revealed that the serum level of IL-4 was significantly higher in patients with E. histolytica associated with healthy observation collection, while IFN-γ showed no significant difference.