Author/Authors :
Simonian, Miganoosh Department of Medical Biotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran, Iran , Haji Ghaffari, Mozhan Department of Medical Biotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran, Iran , Salimi, Ali Monoclonal Antibody Research Center - Avicenna Research Institute - ACECR, Tehran, Iran , Mirzadegan, Ebrahim Nanobiotechnology Research Center - Avicenna Research Institute - ACECR, Tehran, Iran , Sadeghi, Niloufar Monoclonal Antibody Research Center - Avicenna Research Institute - ACECR, Tehran, Iran , Ebrahimnezhad, Nasim Monoclonal Antibody Research Center - Avicenna Research Institute - ACECR, Tehran, Iran , Fazli, Ghazaleh Monoclonal Antibody Research Center - Avicenna Research Institute - ACECR, Tehran, Iran , Fatemi, Ramina Monoclonal Antibody Research Center - Avicenna Research Institute - ACECR, Tehran, Iran , Bayat, Ali-Ahmad Monoclonal Antibody Research Center - Avicenna Research Institute - ACECR, Tehran, Iran , Milani, Saeideh Blood Transfusion Research Center - High Institute for Research and Education in Transfusion Medicine, Tehran, Iran , Negahdari, Babak Department of Medical Biotechnology - School of Advanced Technologies in Medicine - Tehran University of Medical Sciences, Tehran, Iran , Rabbani, Hodjattallah Monoclonal Antibody Research Center - Avicenna Research Institute - ACECR, Tehran, Iran
Abstract :
Background: Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells.
Methods: In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines.
Results: Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, re-spectively. The immunocytochemistry staining results confirmed sortilin surface expres-sion. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines.
Conclusion: Our study revealed the important role of surface sortilin in breast carci-noma cell survival and its possible application as a therapeutic agent in cancer tar-geted therapies.
