Author/Authors :
Moayeri, Ardeshir Department of Anatomy - Faculty of Medicine - Ilam University of Medical Sciences, Ilam, Iran , Alizadeh, Rafieh ENT and Head & Neck Research Center and Department - The Five Senses Institute - Iran University of Medical Sciences, Tehran, Iran , Ghasemi Hamidabadi, Hatef Department of Anatomy & Cell Biology - Faculty of Medicine - Mazandaran University of Medical Sciences, Sari, Iran - Immunogenetic Research Center - Department of Anatomy & Cell Biology - Faculty of Medicine - Mazandaran University of Medical Sciences, Sari, Iran , Nazm Bojnordi, Maryam Department of Anatomy & Cell Biology - Faculty of Medicine - Mazandaran University of Medical Sciences, Sari, Iran - Immunogenetic Research Center - Department of Anatomy & Cell Biology - Faculty of Medicine - Mazandaran University of Medical Sciences, Sari, Iran , Niapour, Ali Department of Anatomical Sciences - Research Laboratory for Embryology and Stem Cells - School of Medicine - Ardabil University of Medical Sciences, Ardabil, Iran , Hedayatpour, Azim Deptartment of Anatomy - School of Medicine - Medical Sciences/University of Tehran, Tehran, Iran , Darvishi, Marzieh Department of Anatomy - Faculty of Medicine - Ilam University of Medical Sciences, Ilam, Iran , Heidari, Fatemeh Anatomy Department - Faculty of Medicine - Qom University of Medical Sciences, Qom, Iran , Soleimani, Maryam Department of Basic Sciences - University of Social Welfare and Rehabilitation Sciences, Tehran, Iran , Elyasi, Leila Department of Anatomy - Neuroscience Research Center - Faculty of Medicine - Golestan University of Medical Sciences, Gorgan, Iran
Abstract :
Introduction: The induction of from human umbilical cord-derived mesenchymal stem cells (HUC-MSCs) toward dopaminergic neurons is a major challenge in tissue engineering and experimental and clinical treatments of various neurodegenerative diseases including Parkinson’s disease. This study aimed to differentiate HUC-MSCs into dopaminergic neuron-like cells.
Methods: After HUC-MSCs isolation and characterizations, they transferred onto matrigel- coated plates and incubated with a cocktail of dopaminergic neuronal differentiation factors. The capacity of differentiation into dopaminergic neuron-like cells in 2D culture and on matrigel was assessed by real-time PCR, immunocytochemistry and high-performance liquid chromatography (HPLC).
Results: Our results showed that the transcript and protein levels of dopaminergic neuronal markers was significantly increased on Matrigel differentiated cells as compared with 2D culture plates.
Conclusions: Overall, the results of this study suggest that HUC-MSCs can successfully differentiate toward dopaminergic neuron-like cells on Matrigel, having great potentials for the treatment of dopaminergic neuron-related diseases.
Keywords :
Differentiation , Dopaminergic Neurons , Human Umbilical Cord Mesenchymal Stem Cells , In Vitro
