Author/Authors :
Salam Karim, Y Department of Pharmacy - Al-Manara College for Medical Sciences, Maysan, Iraq , Hachim, S. K College of Technical Engineering - The Islamic University, Najaf, Iraq - Medical Laboratory Techniques Department - Al-Turath University College, Baghdad, Iraq , Abdul Ali, A Education College - Al-Farahidi University, Baghdad, Iraq , Ameen Baqer, A Medical Laboratory Techniques Department - Dijlah University College, Baghdad, Iraq , Ali Yaseen, M Medical Laboratory Techniques Department - AL-Mustaqbal University College, Babylon, Iraq , Lafta, H. A Department of Pharmacy - Al-Nisour University College, Baghdad, Iraq , Hussein Adhab, Z Department of Pharmacy - Al-Zahrawi University College, Karbala, Iraq , Ayad Kareem, H Altoosi University College, Najaf, Iraq , Shaker Hamza, I Al-Esraa University College, Baghdad, Iraq , Hamad, D. A Nursing Department - Hilla University College, Babylon, Iraq
Abstract :
It is well documented that choline is known as one of the essential ingredients of phospholipids. Choline acts as a determinative element for appropriate cell membrane functions. On the other hand α-tocopherol (Vit E) is a fat-soluble vitamin. This vitamin acts as a strong antioxidant in the living body's defense system against
oxidative stress. Lipid peroxidation in peripartum and early lactating cows is significantly increased while the
level of serum Vit E is decreases dramatically. These concomitant physiological changes demonstrate a higher
level of oxidative stress subsequently leads to serious health issues in dairy cows. Therefore, the present
research was designed to investigate the following items in dairy cattle: 1) evaluation of the possible changes in
serum protein fractions, and 2) comparing the oxidative status of orally RPC and vitamin E supplementation in
dairy cows in early lactation period. In the current study 30 early lactating primiparous and multiparous Holstein
cows (body condition score (BCS)=2.51 ± 0.10) were used beginning five weeks postpartum. All the animals
were randomly divided in to three groups (n=10) (number of lactation=2.61). The animals were randomly
assigned to receive one of the following treatments. Group 1 served as control group were not received any
supplement. The second group was supplemented with 90 g/d of RPC (Reashre Choline, Balchem, USA). The
third group was administrated 4400 IU/d vitamin E (Roche, Vitamins Ltd; Switzerland). In the current study,
serum protein electrophoresis showed four main fractions as follows: albumin, α-globulin, β-globulin, and γ-
globulin. The recorded data showed that the percentages of albumin and γ-globulin fractions were higher in
treated groups compared to the control group. In the animals supplementing with RPC and vitamin E the
percentages of serum albumin increased to the value of 37. 70±1.63 and 38.21±1.28 respectively compare to the control group (34.69±1.21), which were significant (P<0.05).
Keywords :
electrophoresis , serum proteins , RPC , vitamin E
