Author/Authors :
Qasim, M. T Department of Anesthesia - College of Health and Medical Technology - Al-Ayen University, Thi-Qar, Iraq , Mahdi Mohammed Alakkam, E Department of Obstetrics and Gynecology - Specialist Obstetrician and Gynecologist - Al-Forat General Hospital - Baghdad, Iraq , Mahdi Mohammed, M Al-Manara College FOR Medical Sciences, Maysan, Iraq , Hachim, S. K Medical Laboratory Techniques Department - Al-Farahidi University, Baghdad, Iraq - Medical Laboratory Techniques Department - Al-Turath University College, Baghdad, Iraq , Sabah Jabr, H Anesthesia Techniques Department - Al-Mustaqbal University College, Babylon, Iraq , Emad Izzat, S Al-Nisour University College, Baghdad, Iraq , Mohammed, K. A Department of Medical Physics - Hilla University College, Babylon, Iraq , Hamood, S. A Al-Esraa University College, Baghdad, Iraq , Shnain Ali, M Department of Dentistry - Al-Zahrawi University College, Karbala, Iraq , Najd Obaid, F Altoosi University College, Najaf, Iraq , Mohsin Al-Dhalemi, D The Islamic University, Najaf, Iraq
Abstract :
Sheep husbandry is considered one of the most important activities in the socio-economic development in the Middle East region, especially in Iraq and Islamic Republic of Iran (IRI). Therefore this study was designed to evaluate the level of ovine pasteurellosis vaccine protective antibody titer and identification of the prevailing serotypes in Iraq (Basrah, Baghdad, Tikrit, Mosul, Erbil). The vaccine was made from pasteurella multosida
Bio-type A and the serotypes of Mannheimia haemolytica. This investigation was performed from September
2021 to January 2022, in Iraq. Sheep blood sera samples were obtained from control unvaccinated and
vaccinated sheep after 14, 21 and 28 days post vaccination. The results showed that out of 319 sheep blood sera
samples which were evaluated using indirect Haemagglutination (IHA) test to detect Mannheimia haemolytica
serotypes, the high prevalence (100 %) of M. haemolytica A2 was found in all the five study regions area, while
96.5 % was M. haemolytica A7 and 88.1 % was M. haemolytica A1. The level of antibody titer was measured
by specific serum antibody titer of pasteurella multosida Bio-type A. The results revealed that out of 268
vaccinated blood sera samples the overall antibody titer were 12 (3.8 %), 16 (5%) and 17 (5.3 %) for protective
antibody titer of 1:160, 1:80 and 1:40 respectively and for antibody titer of 1:20 were 15 (4.7%) and for
antibody titer of 1:10 were 17 (5.3 %), whereas the antibody titer in the control group was 4 (7.8 %). The result
of this study indicated that the vaccine administered has limited protective power against pasteurella multocida
Bio-type A which lead to researchers for further study on identification of specific strain of pasteurella
multosida and development of multivalent vaccine including the most prevalent pasteurella serotypes.
