Title of article :
Differential Expression Pattern of linc-ROR Spliced Variants in Pluripotent and Non-Pluripotent Cell Lines
Author/Authors :
Mirzadeh Azad ، Fatemeh Molecular Genetics Department - Faculty of Biological Sciences - Tarbiat Modares University , Taheri Bajgan ، Elham Molecular Genetics Department - Faculty of Biological Sciences - Tarbiat Modares University , Naeli ، Parisa Molecular Genetics Department - Faculty of Biological Sciences - Tarbiat Modares University , Rudov ، Alexander Department of Biomolecular Sciences - University of Urbino , Bagheri Moghadam ، Mahrokh Cardiogenetic Research Center, Rajaie Cardiovascular Medical and Research Center - Iran University of Medical Sciences , Akhtar ، Mozhgan Sadat Molecular Genetics Department - Faculty of Biological Sciences - Tarbiat Modares University , Gholipour ، Akram Department of Biology - Islamic Azad University, Science and Research branch , Mowla ، Javad Molecular Genetics Department - Faculty of Biological Sciences - Tarbiat Modares University , Malakootian ، Mahshid Cardiogenetic Research Center, Rajaie Cardiovascular Medical and Research Center - Iran University of Medical Sciences
Abstract :
Objective: The human large intergenic non-coding RNA-regulator of reprogramming program (linc-ROR) is known as a stem cell specific linc-RNA. linc-ROR counteracts differentiation via sequestering microRNA-145 (miR-145) that targets OCT4 transcript. Despite the research on the expression and function, the exact structure of Linc-ROR transcripts is not clear. Considering the contribution of alternative splicing in transcripts structures and function, identifying different spliced variants of linc-ROR is necessary for further functional analyses. We aimed to find the alternatively spliced transcripts of linc-ROR and investigate their expression pattern in stem and cancer cell lines and during neural differentiation of NT2 cells as a model for understanding linc-ROR role in stem cell and differentiation. Materials and Methods: In this experimental study, linc-ROR locus was scanned for identifying novel exons. Different primer sets were used to detect new spliced variants by reverse transcription polymerase chain reaction (RT-PCR) and direct sequencing. Quantitative PCR (qPCR) and RT-PCR were employed to profile expression of linc-ROR transcripts in different cell lines and during neural differentiation of stem cells. Results: We could discover 13 novel spliced variants of linc-ROR harboring unique array of exons. Our work uncovered six novel exons, some of which were the product of exonized transposable elements. Monitoring expression profile of the linc-ROR spliced variants in a panel of pluripotent and non-pluripotent cells exhibited that all transcripts were primarily expressed in pluripotent cells. Moreover, the examined linc-ROR spliced variants showed a similar downregulation during neural differentiation of NT2 cells. Conclusion: Altogether, our data showed despite the difference in the structure and composition of exons, various spliced variants of linc-ROR showed similar expression pattern in stem cells and through differentiation.
Keywords :
Linc , ROR , Pluripotency , spliced variants , Stem Cell
Journal title :
Cell Journal (Yakhteh)
Journal title :
Cell Journal (Yakhteh)
