the Effect of Varied Culture Conditions and Nutritional Requirements in the Production of Antimicrobial Metabolite by Streptomyces

Background: This study evaluated the effect of varied culture conditions and nutritional requirements in producing antimicrobial metabolite by Streptomyces. Methods: Streptomyces species were isolated from soil samples using the spread plate method on Streptomyces agar. In addition, the yeast-malt broth was used for fermentation and optimization. The different culture conditions were pH, mineral salt concentrations, and agitation rate. The tested nutritional components were Carbon and Nitrogen sources. Results: We observed the maximum production of the antimicrobial metabolite using the medium containing Galactose anhydride showing high inhibition zone of 32 mm (Staphylococcus aureus) and 23 mm (Pseudomonas aeruginosa), respectively. Maximum secondary metabolite productivity was also achieved with the medium containing malt extracts Staphylococcus aureus (30 mm) and Pseudomonas aeruginosa (27 mm). For pH, maximum metabolite production was obtained in the medium adjusted to 10 (30 mm, 33 mm). The best metabolite production was obtained at 0.30 mol/ml (30 mm, 27 mm) with the medium incorporated with additional sodium chloride. Good antimicrobial metabolite production was also observed with a medium and maximum agitation speed of 250 rpm. Conclusion: For efficient production of antimicrobial metabolite by Streptomyces species, Galactose anhydride, malt extract, sodium chloride concentration of 0.30 mol/ml, pH of 10, and agitation speed of 250 rpm should be used.